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Profiling of polychromatic flow cytometry data on B-cells reveals patients' clusters in common variable immunodeficiency
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SYSNO ASEP 0335460 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Profiling of polychromatic flow cytometry data on B-cells reveals patients' clusters in common variable immunodeficiency Title Klastrování pacientů s běžným variabilním imunodeficitem na základě profilování dat získaných imunofenotypizací B buněk pomocí polychromatické průtokové cytometrie Author(s) Kalina, T. (CZ)
Stuchlý, J. (CZ)
Janda, A. (CZ)
Hrušák, O. (CZ)
Růžičková, Šárka (BTO-N) RID
Šedivá, A. (CZ)
Litzman, J. (CZ)
Vlková, M. (CZ)Source Title Cytometry. Part A. - : Wiley - ISSN 1552-4922
Roč. 75, č. 11 (2009), s. 902-909Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords binning ; B lymphocyte profiling ; common variable immunodeficiency Subject RIV EC - Immunology CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000271498500003 DOI 10.1002/cyto.a.20801 Annotation New CD21-CD38-CD27-CD24+IgM+ and CD21-CD38- CD27-CD24-IgM+ subpopulations of B lymphocytes were shown using molecular analysis to display the signs of antigen-driven selection, affinity maturation and isotype switching in both patients with immunodeficiences and controls. These populations can be easily identified using so-called binning algorithm allowing generation of clusters of patients and B lymphocyte subsets. The validity of this method was verified using classical flow cytometry gating and correlation between flow cytometry data and clinical parameters Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2010
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