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Polymethacrylate Monolithic and Hybrid Particle-Monolithic Columns for Reversed-Phase and Hydrophilic Interaction Capillary Liquid Chromatography

SYSNO ASEP	0335333
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Polymethacrylate Monolithic and Hybrid Particle-Monolithic Columns for Reversed-Phase and Hydrophilic Interaction Capillary Liquid Chromatography
Title	Polymetakrylátové monolitické a hybridní částicové monolitické kolony pro kapilární kapalinovou chromatografii v systému s obrácenými fázemi a chromatografii hydrofilních interakcí
Author(s)	Jandera, P. (CZ) Urban, J. (CZ) Škeříková, V. (CZ) Langmeier, P. (CZ) Kubíčková, R. (CZ) Planeta, Josef (UIACH-O)_{RID, ORCID}
Number of authors	6
Source Title	Journal of Chromatography A. - : Elsevier - ISSN 0021-9673 Roč. 1217, č. 1 (2010), s. 22-33
Number of pages	12 s.
Country	US - United States
Language	eng - English
Country	NL - Netherlands
Keywords	particle-monolithic columns ; hybrid columns ; polymethacrylate monoliths ; capillary HPLC ; HILIC ; proteins ; phenolic acids
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	GA203/08/1536 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40310501 - UIACH-O (2005-2011)
UT WOS	000273623100004
DOI	1016/j.chroma.2009.09.041
Annotation	We prepared hybrid particle-monolithic polymethacrylate columns for micro-HPLC by in situ polymerization in fused silica capillaries pre-packed with 3–5m C18 and aminopropyl silica bonded particles, using polymerization mixtures based on laurylmethacrylate–ethylene dimethacrylate (co)polymers for the reversed-phase (RP) mode and [2-(methacryloyloxy)ethyl]- dimethyl-(3-sulfopropyl) zwitterionic (co)polymers for the hydrophilic interaction (HILIC) mode. The hybrid particle-monolithic columns showed reduced porosity and hold-up volumes, approximately 2– 2.5 times lower in comparison to the pure monolithic columns prepared in the whole volume of empty capillaries. The elution volumes of sample compounds are also generally lower in comparison to packed or pure monolithic columns. The efficiency and permeability of the hybrid columns are intermediate in between the properties of the reference pure monolithic and particle-packed columns.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2011

Number of the records: 1