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Insects Pathogens: Molecular Approaches and Techniques
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SYSNO ASEP 0335280 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Identification and Diagnostics of Entomopathogenic Protozoa Author(s) Oborník, Miroslav (BC-A) RID, ORCID Source Title Insects Pathogens: Molecular Approaches and Techniques. - Wallingford : CAB International, 2009 / Stock S. P. ; Vandenberg J. ; Glazer I. ; Boemare N. - ISBN 978-1-84593-478-1 Pages s. 101-128 Number of pages 28 s. Number of pages 432 Language eng - English Country GB - United Kingdom Keywords entomopathogenic protozoa ; diagnostics ; strains specific PCR Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) Annotation Molecular methods allow us to recognize and identify target organisms from a variety of environments including laboratory cultures, host tissues or environmental samples. Ascending progress on the field of molecular evolution during last 15 years greatly influenced high order taxonomy. It has been shown that the proposed kingdom Protozoa comprising unicellular heterotrophic eukaryotes is polyphyletic in its nature and its taxonomic relevance is no longer sustainable. Here I show the two up-to-date proposals dividing eukaryotic organisms into five and six major groups (kingdoms) respectively. These are currently the most accepted views on protist and eukaryotic high order taxonomy referred by Simpson and Roger (2004) and Keeling et al., (2005). This chapter follows methods for molecular identification of Protozoa colonizing insects and their molecular diagnostics. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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