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Species identification in the trematomid family using nuclear genetic markers
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SYSNO ASEP 0335258 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Species identification in the trematomid family using nuclear genetic markers Author(s) Van de Putte, A. P. (BE)
Van Houdt, J. K. J. (BE)
Maes, G. E. (BE)
Janko, Karel (UZFG-Y) RID, ORCID
Koubbi, P. (FR)
Rock, J. (GB)
Volckaert, F. A. M. (BE)Source Title Polar Biology. - : Springer - ISSN 0722-4060
Roč. 32, č. 12 (2009), s. 1731-1741Number of pages 11 s. Language eng - English Country DE - Germany Keywords Southern Ocean ; Trematomidae ; Microsatellites Subject RIV EG - Zoology R&D Projects KJB600450602 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KJB600450903 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000271736700004 DOI 10.1007/s00300-009-0672-8 Annotation The Trematominae, a subfamily of the Nototheniidae, are typical of the high-Antarctic shelf waters. Within the Trematominae examples of phenotypic plasticity and possible cryptic speciation have been observed. Morphological identification of adult stages can be problematic in cases of high phenotypic plasticity or cryptic speciation. Additionally, postlarval and juvenile stages often have traits still under development and which lack distinction. A microsatellite DNA multiplex of six markers has been developed for Trematomus newnesi (Van Houdt et al., 2006). This multiplex was tested on five additional trematomid taxa: Pagothenia borchgrevinki, Trematomus bernacchii, T. eulepidotus, T. hansoni and T. scotti. We used these six microsatellite loci to assess the genetic differentiation among species and the resolution power of these loci for individual-based assignment methods. The six species could be well discriminated by conventional methods such as principal component analysis Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
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