Number of the records: 1
Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera
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SYSNO ASEP 0334950 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera Title SELEKTIVNÍ KULTIVACE VYBRANÝCH NOVÝCH DRUHŮ TRYPANOSOMATID IZOLOVANÝCH Z HETEROPTER Author(s) Yurchenko, V. Y. (US)
Lukeš, Julius (BC-A) RID, ORCID
Jirků, Milan (BC-A) RID
Maslov, D. A. (US)Source Title International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society - ISSN 1466-5026
Roč. 59, č. 43 (2009), s. 893-909Number of pages 17 s. Language eng - English Country GB - United Kingdom Keywords gGAPDH ; maximum-likelihood ; spliced leader RNA ; small-subunit rRNA ; trypanosomatids Subject RIV EB - Genetics ; Molecular Biology R&D Projects 2B06129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC07032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000265901700047 DOI 10.1099/ijs.0.001149-0 Annotation Mixed trypanosomatid infections have long been suspected to represent an obstacle for recovering cultures that would faithfully represent original species descriptions. However, without the means to directly compare the parasites in the host and in culture, this would remain just a possibility. Here we have used PCR-based genotyping of spliced leader RNA gene repeats to analyse several novel species of insect trypanosomatids isolated from heteropteran hosts and to compare them with the parasites that had been detected in the gut smears of the same hosts. We have found that most of the axenic cultures contained novel species of Crithidia and Leptomonas. Therefore, we concluded that, in each case, this replacement was caused by differences in cultivation properties between the original predominant blastocrithidia and the less fastidious parasite that was later recovered in culture. The properties of the new organisms were investigated and used to describe five novel species. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
Number of the records: 1