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Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic

SYSNO ASEP	0334941
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic
Author(s)	Ondráčková, Z. (CZ) Kváč, Martin (BC-A)_{RID, RID, ORCID} Sak, Bohumil (BC-A)_{RID, ORCID} Květoňová, Dana (BC-A)_SAI Rost, M. (CZ)
Source Title	Veterinary Parasitology. - : Elsevier - ISSN 0304-4017 Roč. 165, 1/2 (2009), s. 141-144
Number of pages	4 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Cryptosporidium spp. ; cattle ; slaughterhouses
Subject RIV	GJ - Animal Vermins ; Diseases, Veterinary Medicine
R&D Projects	GP523/07/P117 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000271160200021
DOI	10.1016/j.vetpar.2009.06.035
Annotation	A total of 995 faecal samples from slaughtered dairy cattle was examined microscopically for the presence of Cryptosporidium spp. Nested PCR amplifying the partial SSU rRNA and GP60 genes of Cryptosporidium was performed on all positive and 200 randomly selected samples and the products were digested by SspI, VspI and MboII restriction enzymes. The highest prevalence of infection (18.2%) was in the animals in age group of 12-18 months. The sequence analyses of PCR-positive specimens identified 41 samples as C. andersoni (4.1%), 2 samples as C. bovis (0.2%), and 1 sample as C. parvum (0.1%). Cryptosporidium bovis was detected only in a group of cattle older than 18 months and C. parvum in heifer which was older than 14 months. Infection intensity was generally low. Sequence analysis of the GP60 gene showed that detected C. parvum belonged to the IIaA16G1R1 subtype. This is the first published report about subtyping of the Cryptosporidium GP60 gene from cattle in the Czech Republic.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2010

Number of the records: 1