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Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide effective tool for their molecular phylogenetics

SYSNO ASEP	0334902
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide effective tool for their molecular phylogenetics
Title	Spojená SSU a LSU rDNA data potvrzují hlavní evoluční směry myxosporeí (Myxozoa: Myxosporea) a poskytují účinný nástroj pro rekonstrukci jejich fylogeneze
Author(s)	Bartošová, Pavla (BC-A)_{RID, ORCID} Fiala, Ivan (BC-A)_{RID, ORCID} Hypša, Václav (BC-A)_RID
Source Title	Molecular Phylogenetics and Evolution. - : Elsevier - ISSN 1055-7903 Roč. 53, č. 1 (2009), s. 81-93
Number of pages	12 s.
Language	eng - English
Country	US - United States
Keywords	myxosporea ; phylogeny ; LBA ; LSU rDNA ; 28S ; SSU rDNA ; 18S ; D domains
Subject RIV	EG - Zoology
R&D Projects	KJB600960701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC522 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000269067900008
DOI	10.1016/j.ympev.2009.05.018
Annotation	Views on myxosporean phylogeny and systematics have recently undergone substantial changes resulting from analyses of SSU rDNA. Here, we further investigate the evolutionary trends within myxosporean lineages by using 35 new sequences of the LSU rDNA. We show a good agreement between the two rRNA genes and confirm the main phylogenetic split between the freshwater and marine lineages. The informative superiority of the LSU data is shown by an increase of the resolution, nodal supports and tree indexes in the LSU rDNA and combined analyses. We determine the most suitable part of LSU for the myxosporean phylogeny by comparing informative content in various regions of the LSU sequences. Based on this comparison, we propose the D5–3′-end part of the LSU rRNA gene as the most informative region which provides in concatenation with the complete SSU a well resolved and robust tree. To allow for simple amplification of the marker, we design specific primer set for this part of LSU rDNA.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2010

Number of the records: 1