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Novel genes retrieved from environmental DNA by polymerace chain reaction: current genome-walking techniques for future metagenome applications
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SYSNO ASEP 0334836 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Novel genes retrieved from environmental DNA by polymerace chain reaction: current genome-walking techniques for future metagenome applications Title Získání nových genů z environmentální DNA pomocí PCR: současné techniky „genome walking“ pro budoucí metagenomické aplikace Author(s) Kotík, Michael (MBU-M) RID, ORCID Source Title Journal of Biotechnology. - : Elsevier - ISSN 0168-1656
Roč. 144, č. 2 (2009), s. 75-82Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords Metagenome ; Consensus sequence ; Genome walking Subject RIV EE - Microbiology, Virology R&D Projects GA204/06/0458 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000272100900001 DOI 10.1016/j.jbiotec.2009.08.013 Annotation Environmental DNA is an extremely rich source of genes encoding enzymes with novel biocatalytic activities. This review covers the diverse array of genome-walking techniques, which were originálky developed for genomic DNA and currently are also used for PCR-based recovery of entire genes from the metagenome Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2010
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