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Confocal microscopy reveals Myzitiras and Vthela morphotypes as new signatures of malignancy progression
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SYSNO ASEP 0334133 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Confocal microscopy reveals Myzitiras and Vthela morphotypes as new signatures of malignancy progression Author(s) Veselý, Pavel (UMG-J)
Rösel, D. (CZ)
Paňková, D. (CZ)
Tolde, O. (CZ)
Blase, C. (DE)
Matoušková, E. (CZ)
Folk, P. (CZ)
Brábek, J. (CZ)
Bereiter-Hahn, J. (DE)Number of authors 9 Source Title Scanning - ISSN 0161-0457
Roč. 31, č. 3 (2009), s. 102-106Number of pages 5 s. Language eng - English Country US - United States Keywords dynamic morphology ; cinemicroscopy ; breast carcinoma ; malignancy progression Subject RIV EB - Genetics ; Molecular Biology R&D Projects LC06061 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000268426100002 DOI 10.1002/sca.20146 Annotation G3S1 cells are a new line derived from EM-G3 breast cancer cells by chronic nutritional stress and treatments with 12-O-tetradecanoylphorbol-13-acetate. These cells are capable of growing in standard medium. G3S1 cells exhibited elevated invasiveness in Matrigel invasion chambers as compared with parental EM-G3 cells. Elevated invasiveness of G3S1 cells was accompanied by higher incidence of myzitiras morphotype (sucker-like) and newly observed vthela morphotype (leech-like) both inducible in Hanks Balanced Salt Solution test. Time-lapse phase contrast microscopy showed a capacity of G3S1 cells to form lobopodial protrusions already 20 min after seeding on gelatin. These protrusions could make contact with the dish and possibly produce the vthela shape. The possible relationship of mysitiras and vthela morphotypes to an increase in malignant potential marked by enhanced invasiveness was thus indicated. Copyright 2009 Wiley Periodicals, Inc. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2010
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