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Spatial and temporal regulation of Wnt/beta-catenin signaling is essential for development of the retinal pigment epithelium
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SYSNO ASEP 0334091 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Spatial and temporal regulation of Wnt/beta-catenin signaling is essential for development of the retinal pigment epithelium Author(s) Fujimura, Naoko (UMG-J)
Taketo, M.M. (JP)
Mori, M. (JP)
Kořínek, Vladimír (UMG-J) RID
Kozmik, Zbyněk (UMG-J) RIDNumber of authors 5 Source Title Developmental Biology. - : Elsevier - ISSN 0012-1606
Roč. 334, č. 1 (2009), s. 31-45Number of pages 15 s. Language eng - English Country US - United States Keywords eye ; Wnt ; retina ; pigment Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/08/1618 GA ČR - Czech Science Foundation (CSF) 1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 2B06077 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000272260600004 DOI 10.1016/j.ydbio.2009.07.002 Annotation Wnt/beta-catenin signaling is highly active in the dorsal retinal pigment epithelium (RPE) during eye development. To study the role of Wnt/beta-catenin signaling in the RPE development we used a conditional Cre/loxP system in mice to inactivate or ectopically activate Wnt/beta-catenin signaling in the RPE. Inactivation of Wnt/beta-catenin signaling results in transdifferentiation of RPE to neural retina (NR) as documented by downregulation of RPE-specific markers Mitf and Otx2 and ectopic expression of NR-specific markers Chx10 and Rx, respectively. In contrast, ectopic activation of Wnt/beta-catenin signaling results in the disruption of the RPE patterning, indicating that precise spatial and temporal regulation of Wnt/beta-catenin signaling is required for normal RPE development. Combined, our data suggest that Wnt/beta-catenin signaling plays an essential role in development of RPE by maintaining or inducing expression of Mitf and Otx2. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2010
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