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Recovery of tubulin functions after freeze-dying in the presence of trehalose

SYSNO ASEP	0334042
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Recovery of tubulin functions after freeze-dying in the presence of trehalose
Author(s)	Dráberová, Eduarda (UMG-J)_{RID, ORCID} Sulimenko, Vadym (UMG-J)_{RID, ORCID} Sulimenko, Tetyana (UMG-J) Böhm, K. J. (DE) Dráber, Pavel (UMG-J)_{RID, ORCID}
Number of authors	5
Source Title	Analytical Biochemistry. - : Elsevier - ISSN 0003-2697 Roč. 397, č. 1 (2010), s. 67-72
Number of pages	6 s.
Language	eng - English
Country	US - United States
Keywords	Tubulin ; Freeze-drying ; Stability
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	KAN200520701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000273883700009
DOI	10.1016/j.ab.2009.10.016
Annotation	We describe a novel method for freeze-drying of assembly-competent tubulin in the presence of a nonreducing sugar trehalose. Even after prolonged storage at ambient temperature, rehydrated tubulin is capable of binding antimitotic drugs and assembling to microtubules that bind microtubule-associated proteins in the usual way.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2010

Number of the records: 1