TRAIL-induced apoptosis of HL60 leukemia cells: Two distinct phenotypes of acquired TRAIL resistance that are accompanied with resistance to TNFa but not to idarubicin and cytarabine

Klener, P.Jr.; Leahomschi, S.; Molinsky, J.; Simonova, T.; Nečas, E.; Gašová, Z.; Čermák, J.; Doležalová, L.; Anděra, Ladislav; Živný, J.

doi:https://dx.doi.org/10.1016/j.bcmd.2008.10.002

Number of the records: 1

TRAIL-induced apoptosis of HL60 leukemia cells: Two distinct phenotypes of acquired TRAIL resistance that are accompanied with resistance to TNFa but not to idarubicin and cytarabine

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SYSNO ASEP	0334040
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	TRAIL-induced apoptosis of HL60 leukemia cells: Two distinct phenotypes of acquired TRAIL resistance that are accompanied with resistance to TNFa but not to idarubicin and cytarabine
Author(s)	Klener, P.Jr. (CZ) Leahomschi, S. (CZ) Molinsky, J. (CZ) Simonova, T. (CZ) Nečas, E. (CZ) Gašová, Z. (CZ) Čermák, J. (CZ) Doležalová, L. (CZ) Anděra, Ladislav (UMG-J)_RID Živný, J. (CZ)
Number of authors	10
Source Title	Blood Cells Molecules and Diseases. - : Elsevier - ISSN 1079-9796 Roč. 42, č. 1 (2009), s. 77-84
Number of pages	8 s.
Language	eng - English
Country	US - United States
Keywords	TRAIL resistance ; leukemia ; therapy
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000262347900014
DOI	10.1016/j.bcmd.2008.10.002
Annotation	TNF-related apoptosis-inducing ligand (TRAIL) is a proapoptotic cytokine implicated in cancer cell surveillance. Prolonged exposure of TRAIL-sensitive leukemia cell line HL60 cells to recombinant TRAIL or to cytostatic agents, cytarabine and idarubicin, resulted in the establishment of resistant subclones with distinct phenotypes. The TRAIL resistant subclones had decreased expression of TRAIL and TNFa death receptors, impaired activation of caspases 8 and 10 in response to TRAIL and TNFa and dysregulated expression of several apoptosis regulators. TRAIL resistant HL60 showed two phenotypes with different expression of CD14, osteoprotegerin, and several apoptosis regulators and were resistant to TNFa, suggesting disruption of the extrinsic apoptotic pathway. The concurrently derived cytarabine and idarubicin-resistant HL60 subclones remained sensitive to TRAIL-induced apoptosis. We identified distinct pathways for the development of HL60 leukemia cell resistance to apoptosis induction.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2010

Number of the records: 1