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Controlled dehydration of a biological sample using an alternative form of environmental SEM
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SYSNO ASEP 0333986 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Controlled dehydration of a biological sample using an alternative form of environmental SEM Author(s) Neděla, Vilém (UPT-D) RID, ORCID, SAI Number of authors 1 Source Title Journal of Microscopy. - : Wiley - ISSN 0022-2720
Roč. 237, č. 1 (2010), s. 7-11Number of pages 5 s. Language eng - English Country GB - United Kingdom Keywords biological sample ; dehydration ; environmental SEM ; AQUASEM II ; hydration system Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering CEZ AV0Z20650511 - UPT-D (2005-2011) UT WOS 000272659400002 EID SCOPUS 72349088450 DOI 10.1111/j.1365-2818.2009.03216.x Annotation In this study a non-conductive biological sample is observed free of charging artefacts when placed on a cooled Peltier stage in the specimen chamber of an alternative form of the environmental scanning electron microscope, equipped with a specially designed hydration system. This system was used to create dynamically changing surrounding conditions leading to controlled dehydration of the sample enabling us to visualize the topographical structure of a rat tongue in the transition region between the liquid and the gas state of water in the microscope specimen chamber. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2010
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