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The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence

SYSNO ASEP	0333814
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence
Title	Vliv detergentů na aktivitu trimerních G-proteinů v plasmatických membránách izolovaných z mozkové kůry: korelace s fluorescencí DPH a Laurdanu
Author(s)	Sýkora, Jan (UFCH-W)_RID Bouřová, Lenka (FGU-C) Hof, Martin (UFCH-W)_{RID, ORCID} Svoboda, Petr (FGU-C)_{RID, ORCID}
Source Title	Biochimica Et Biophysica Acta-Biomembranes. - : Elsevier - ISSN 0005-2736 Roč. 1788, č. 2 (2009), s. 324-332
Number of pages	9 s.
Language	eng - English
Country	NL - Netherlands
Keywords	detergent ; fluorescence ; stready-state ; time-resolved
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LC554 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA309/06/0121 GA ČR - Czech Science Foundation (CSF)
	IAA500110606 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
	AV0Z50110509 - FGU-C (2005-2011)
UT WOS	000263794700004
DOI	10.1016/j.bbamem.2008.11.008
Annotation	The effect of non-ionic detergents on baclofen (GABA(B)-R agonist)-stimulated G-protein activity was measured as a [S-35] GTP gamma S,IS binding assay in the plasma membranes (PM) isolated from the brain tissue. The effect was clearly biphasic - a decrease in the activity was followed by an activation maximum and finally, at high concentrations, drastic inhibition of the G-protein activity was noticed. Contrarily, specific radioligand binding to GABA(B)-receptor was inhibited in the whole range of detergent concentrations step by step, i.e. it was strictly monophasic. The magnitude of both detergent effects was decreased in the same order of potency: Brij58>Triton X-100>Digitonin. The identical order was found when comparing detergents ability to alter fluorescence anisotropy of the membrane probe 1,6-diphenyl-1,3,5-hexatriene (r (DPH)) incorporated into the hydrophobic PM interior.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2010

Number of the records: 1