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A comparative study on ganglioside micelles using electronic energy transfer, fluorescence correlation spectroscopy and light scattering techniques
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SYSNO ASEP 0333803 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A comparative study on ganglioside micelles using electronic energy transfer, fluorescence correlation spectroscopy and light scattering techniques Title Studie micel tvořených GM1 gangliosidy za použití elektronického přenosu energie, fluorescenční korelační spektroskopie a rozptylu světla Author(s) Šachl, Radek (UFCH-W) RID, ORCID
Mikhalyov, I. (RU)
Hof, Martin (UFCH-W) RID, ORCID
Johansson, L. B. A. (SE)Source Title Physical Chemistry Chemical Physics. - : Royal Society of Chemistry - ISSN 1463-9076
Roč. 11, č. 21 (2009), s. 4335-4343Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords atomic-force microscopy ; Alzheimers disease ; precise measurement Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GD203/05/H001 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000266269200025 DOI 10.1039/b821658d Annotation Ganglioside (G(M1)) micelles have been studied by means of three different techniques: fluorescence correlation spectroscopy (FCS), electronic energy transfer, as monitored by time-resolved fluorescence spectroscopy, as well as static and dynamic light scattering. The aggregation numbers obtained, 168 +/- 4, remain constant over a wide range of G(M1) concentrations (0.764-156 mu M), are very consistent when using dfferent donor-acceptor energy transfer pairs and have served as reference values in tests of the FCS method. It is recommended to calibrate the focal volume by using known dye concentrations. For this the rhodamine dye, 5-TAMRA, turns out to be most suitable. It is also shown that FCS provides correct values of the aggregation numbers, provided that the focal volume is calibrated by using updated values of the diffusion constant of Rhodamine 6G. These results also support recent methodological advances in FCS. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2010
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