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Simultaneous Characterization of Lateral Lipid and Prothrombin Diffusion Coefficients by Z-Scan Fluorescence Correlation Spectroscopy

SYSNO ASEP	0333797
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Simultaneous Characterization of Lateral Lipid and Prothrombin Diffusion Coefficients by Z-Scan Fluorescence Correlation Spectroscopy
Title	Charakterizace laterálních difúzních koeficientů lipidů a prothrombinu současně stanovených pomocí metody z-scan ve fluorescenční korelační spektroskopii
Author(s)	Štefl, Martin (UFCH-W) Kulakowska-Zań, Anna (UFCH-W) Hof, Martin (UFCH-W)_{RID, ORCID}
Source Title	Biophysical Journal. - : Cell Press - ISSN 0006-3495 Roč. 97, č. 3 (2009), L01-L04
Number of pages	4 s.
Language	eng - English
Country	US - United States
Keywords	bovine prothrombin ; translational diffusion ; protein-binding
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA203/08/0114 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
UT WOS	000268926500001
DOI	10.1016/j.bpj.2009.05.031
Annotation	A new (to our knowledge) robust approach for the determination of lateral diffusion coefficients of weakly bound proteins is applied for the phosphatidylserine specific membrane interaction of bovine prothrombin. It is shown that z-scan fluorescence correlation spectroscopy in combination with pulsed interleaved dual excitation allows simultaneous monitoring of the lateral diffusion of labeled protein and phospholipids. Moreover, from the dependencies of the particle numbers on the axial sample positions at different protein concentrations phosphatidylserine-dependent equilibrium dissociation constants are derived confirming literature values. Increasing the amount of membrane-bound prothrombin retards the lateral protein and lipid diffusion, indicating coupling of both processes.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2010

Number of the records: 1