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Head and neck squamous cancer stromal fibroblasts produce growth factors influencing phenotype of normal human keratinocytes
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SYSNO ASEP 0333778 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Head and neck squamous cancer stromal fibroblasts produce growth factors influencing phenotype of normal human keratinocytes Author(s) Strnad, Hynek (UMG-J) RID
Lacina, L. (CZ)
Kolář, Michal (UMG-J) RID, ORCID
Čada, Z. (CZ)
Vlček, Čestmír (UMG-J) RID
Dvořánková, B. (CZ)
Betka, J. (CZ)
Plzák, J. (CZ)
Chovanec, M. (CZ)
Šáchová, Jana (UMG-J)
Valach, J. (CZ)
Urbanová, Markéta (UMG-J)
Smetana, K. Jr. (CZ)Number of authors 13 Source Title Histochemistry and Cell Biology. - : Springer - ISSN 0948-6143
Roč. 133, č. 2 (2010), s. 201-211Number of pages 11 s. Language eng - English Country DE - Germany Keywords Cancer microenvironment ; Epithelial–mesenchymal interaction ; Cancer-associated fibroblasts Subject RIV EB - Genetics ; Molecular Biology R&D Projects 2B06106 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000273745800006 DOI 10.1007/s00418-009-0661-6 Annotation Epithelial–mesenchymal interaction between stromal fibroblasts and cancer cells influences the functional properties of tumor epithelium, including the tumor progression and spread. We compared fibroblasts prepared from stroma of squamous cell carcinoma and normal dermal fibroblasts concerning their biological activity toward normal keratinocytes assessed by immunocytochemistry and profiling of gene activation for growth factors/cytokines by microarray chip technology. IGF-2 and BMP-4 were determined as candidate factors responsible for tumor-associated fibroblast activity that influences normal epithelia. This effect was confirmed by addition of recombinant IGF-2 and BMP4, respectively, to the culture medium. This hypothesis was also verified by inhibition experiments where blocking antibodies were employed in the medium conditioned by cancer-associated fibroblast. Presence of these growth factors was also detected in tumor samples. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2010
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