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Molecular variability of FLT3/ITD mutants and their impact on the differentiation program of 32D cells: Implications for the biological properties of AML blasts
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SYSNO ASEP 0333746 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Molecular variability of FLT3/ITD mutants and their impact on the differentiation program of 32D cells: Implications for the biological properties of AML blasts Author(s) Peková, S. (CZ)
Ivánek, Robert (UMG-J)
Dvořák, Michal (UMG-J) RID
Rueggeberg, S. (DE)
Leicht, S. (DE)
Li, X. (DE)
Franz, T. (DE)
Kozak, T. (CZ)
Vrba, J. (CZ)
Koza, V. (CZ)
Karas, M. (CZ)
Schwarz, J. (CZ)
Cetkovský, P. (CZ)
Průcha, M. (CZ)Number of authors 14 Source Title Leukemia Research. - : Elsevier - ISSN 0145-2126
Roč. 33, č. 10 (2009), s. 1409-1416Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords FLT3/ITD ; microarrays ; proteomics Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/06/1728 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000268375500023 DOI 10.1016/j.leukres.2009.01.004 Annotation FLT3 is the most frequently mutated gene in acute myeloid leukemia (AML), with internal tandem duplications (ITDs) accounting for up to 30% of its mutations. To analyze the impact of individual ITDs on the expression profile of immature myeloid cells, we have established 32D cell lines expressing nine different FLT3/ITDs isolated from AML patients and subjected them to whole genome expression profiling and 2DE/LC/MS proteomics. Our data indicate that in comparison to the controls, FLT3/ITD-positive 32D cells exhibit less mature expression profiles resembling early hematopoietic progenitors. Moreover, our results suggest that there exist biological differences among individual ITD variants. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2010
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