Number of the records: 1

Compensation of illumination inhomogeneities in multi-resolution image acquisition in confocal microscopy

SYSNO ASEP	0333693
Document Type	C - Proceedings Paper (int. conf.)
R&D Document Type	Conference Paper
Title	Compensation of illumination inhomogeneities in multi-resolution image acquisition in confocal microscopy
Title	Kompenzace jasových nehomogenit při snímání obrazů konfokální mikroskopií
Author(s)	Tomori, Z. (SK) Čapek, Martin (FGU-C)_{RID, ORCID} Michálek, Jan (FGU-C)_RID Kubínová, Lucie (FGU-C)_{RID, ORCID}
Source Title	GraphiCon´2009. - Moscow : Lomonosov Moscow State University, 2009
Pages	s. 108-111
Number of pages	4 s.
Action	International Conference on Computer Graphics and Vision /19./
Event date	05.10.2009-09.10.2009
VEvent location	Moscow
Country	RU - Russian Federation
Event type	WRD
Language	eng - English
Country	RU - Russian Federation
Keywords	confocal microscopy ; light attenuation ; distribution function
Subject RIV	IN - Informatics, Computer Science
R&D Projects	GA102/08/0691 GA ČR - Czech Science Foundation (CSF)
	MEB080831 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
Annotation	A multi-resolution application in modern confocal microscopy is based on a set of objectives with different resolution and motorized XY stage. An image captured through a low-resolution objective serves as navigation map where the user points to positions or areas of interest in which a high-resolution image will be captured. This allows e.g. automated acquisition in time lapse experiments, generating random, or systematic random positions for stereological counting frames, image tiling etc. However, captured image series representing a volume of interest suffers from illumination inhomogeneities. Algorithms described in this paper compensate both uneven illumination of captured images and light attenuation with depth. The first problem was solved by using modified morphological operation called the upper Lipschitz cover. To compensate the light attenuation with depth, a novel, very fast algorithm based on matching of distribution functions has been proposed
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2010

Number of the records: 1