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Hemoglobin digestion in blood-feeding ticks: mapping of multipeptidase pathway by functional proteomics

SYSNO ASEP	0333131
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Hemoglobin digestion in blood-feeding ticks: mapping of multipeptidase pathway by functional proteomics
Author(s)	Horn, Martin (UOCHB-X)_{RID, ORCID} Nussbaumerová, Martina (UOCHB-X)_RID Šanda, Miloslav (UOCHB-X) Kovářová, Zuzana (UOCHB-X)_{RID, ORCID} Srba, Jindřich (UOCHB-X) Franta, Zdeněk (BC-A) Sojka, Daniel (BC-A)_{RID, ORCID} Bogyo, M. (US) Caffrey, C. R. (US) Kopáček, Petr (BC-A)_{RID, ORCID} Mareš, Michael (UOCHB-X)_{RID, ORCID}
Number of authors	11
Source Title	Chemistry & Biology. - : Elsevier - ISSN 1074-5521 Roč. 16, č. 10 (2009), s. 1053-1063
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	peptidases ; active-site probes ; hemoglobinolysis ; tick digestion ; Ixodes ricinus
Subject RIV	CE - Biochemistry
R&D Projects	GA206/06/0865 GA ČR - Czech Science Foundation (CSF)
	IAA600960910 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC06009 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	KJB400550516 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000271894000008
DOI	10.1016/j.chembiol.2009.09.009
Annotation	Hemoglobin digestion is an essential process for blood-feeding parasites. We deconvoluted the hemoglobinolytic cascade in the tick Ixodes ricinus, a vector of Lyme disease and tick-borne encephalitis. A network of digestive peptidases was demonstrated through imaging with specific activity-based probes and activity profiling. Selective inhibitors were applied to dissect the roles of the individual peptidases and determine the peptidase-specific cleavage map of the hemoglobin molecule. Because of their central function in nutrition of the parasite, the identified enzymes are potential targets to developing novel anti-tick vaccines that limit parasite survival and disease transmission.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2010

Number of the records: 1