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Profiling ABA metabolites in Nicotiana tabacum L. leaves by ultra-performance liquid chromatography–electrospray tandem mass spectrometry

SYSNO ASEP	0332937
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Profiling ABA metabolites in Nicotiana tabacum L. leaves by ultra-performance liquid chromatography–electrospray tandem mass spectrometry
Title	Analýza ABA metabolitů v listech Nicotiana tabacum L. pomocí UPLC-ESI-MS/MS
Author(s)	Turečková, Veronika (UEB-Q)_{RID, ORCID} Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Source Title	Talanta. - : Elsevier - ISSN 0039-9140 Roč. 80, č. 1 (2009), s. 390-399
Number of pages	10 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Abscisic acid ; Ultra-performance liquid chromatography (UPLC) ; Tandem mass spectrometry (MS/MS)
Subject RIV	EC - Immunology
R&D Projects	KAN200380801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000271055700057
DOI	10.1016/j.talanta.2009.06.027
Annotation	We have developed a simple method for extracting and purifying (+)-abscisic acid (ABA) and eight ABA metabolites – before analysis by a novel technique for these substances, ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). The procedure includes addition of deuterium-labelled standards, extraction with methanol-water-acetic acid (10:89:1, v/v), simple purification by Oasis® HLB cartridges, rapid chromatographic separation by UPLC, and sensitive, accurate quantification by MS/MS in multiple reaction monitoring modes. The detection limits of the technique ranged between 0.1 - 1 pmoL for ABAGE and ABA acids in negative ion mode, and 0.01 – 0.50 pmoL for ABAGE, ABAaldehyde, ABAalcohol and the methylated acids in positive ion mode. We isolated ABA and its metabolites from control and water-stressed tobacco leaf tissues. Only ABA, PA, DPA, neoPA, and ABAGE were detected in the samples.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2010

Number of the records: 1