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Nerve Agents Assay Using Cholinesterase Based Biosensor
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SYSNO ASEP 0332920 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nerve Agents Assay Using Cholinesterase Based Biosensor Author(s) Pohanka, M. (CZ)
Dobeš, Petr (UOCHB-X)
Drtinová, L. (CZ)
Kuča, K. (CZ)Number of authors 4 Source Title Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 21, č. 10 (2009), s. 1177-1182Number of pages 6 s. Language eng - English Country DE - Germany Keywords organophosphate ; biosensor ; acetylcholinesterase Subject RIV CF - Physical ; Theoretical Chemistry CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000266537700009 DOI 10.1002/elan.200804528 Annotation Electrochemical biosensor based on electric eel acetylcholinesterase (AChE) (EC 3.1.1.7) was performed for assay of nerve agents – tabun, sarin, soman, cyclosarin, and VX. The biosensor used AChE as biorecognition element. The presence of nerve agents was accompanied by a strong inhibition of AChE activity. Enzyme activity is easily measurable by electrochemical oxidation of thiocholine created from acetylthiocholine (ATChCl) by AChE-catalyzed hydrolysis. The tested nerve agents were successfully assayed. The best limits of detection were achieved for sarin (5.88×10-10 M) and VX (8.51×10-10 M) after one-minute assay. The biosensor was found long term stable at low as well as laboratory temperature. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2010
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