Number of the records: 1

Comparison of HPLC/MS and MALDI-MS for characterizing triacylglycerols in insects: Specie-specific composition of lipids in fat bodies of bumblebee males

SYSNO ASEP	0332852
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Comparison of HPLC/MS and MALDI-MS for characterizing triacylglycerols in insects: Specie-specific composition of lipids in fat bodies of bumblebee males
Author(s)	Kofroňová, Edita (UOCHB-X)_RID Cvačka, Josef (UOCHB-X)_{RID, ORCID} Vrkoslav, Vladimír (UOCHB-X)_{RID, ORCID} Hanus, Robert (UOCHB-X)_{RID, ORCID} Jiroš, Pavel (UOCHB-X) Kindl, Jiří (UOCHB-X)_RID Hovorka, Oldřich (UOCHB-X) Valterová, Irena (UOCHB-X)_{RID, ORCID}
Number of authors	8
Source Title	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232 Roč. 877, č. 30 (2009), s. 3878-3884
Number of pages	7 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Bumblebee ; fat body ; triacylglycerols ; HPLC/APCI-MS ; MALDI-MS
Subject RIV	CC - Organic Chemistry
R&D Projects	GA203/09/0139 GA ČR - Czech Science Foundation (CSF)
	GA203/09/1446 GA ČR - Czech Science Foundation (CSF)
	2B06007 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000271878100014
DOI	10.1016/j.chromb.2009.09.040
Annotation	Two mass spectrometric methods for analysing triacylglycerols (HPLC/APCI-MS and MALDI-MS) were used and compared in terms of the relevance of the data for further biostatistical evaluation. While MALDI-MS is simpler and significantly faster, the time-consuming and labour-intensive HPLC/APCI-MS provides more complete information about the lipid components. The compositions of triacylglycerols in the fat bodies of males in 11 bumblebee species (Bombus terrestris, B. lucorum, B. lapidarius, B. pratorum, B. sylvarum, B. ruderatus, B. pomorum, B. subterraneus, B. campestris, B. bohemicus, and B. rupestris) were found to be species-specific.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2010

Number of the records: 1