Number of the records: 1
Expression of Tumor Necrosis Factor Alpha-Included Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles
- 1.
SYSNO ASEP 0332768 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Expression of Tumor Necrosis Factor Alpha-Included Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles Author(s) Nagyová, Eva (UZFG-Y) RID, ORCID
Němcová, Lucie (UZFG-Y) RID
Procházka, Radek (UZFG-Y) RID, ORCIDSource Title Journal of Reproduction and Development. - : Society for Reproduction and Development - ISSN 0916-8818
Roč. 55, č. 3 (2009), s. 231-235Number of pages 5 s. Language eng - English Country JP - Japan Keywords Preovulatory Ovarian Follicles ; TNFAIP6 ; female fertility Subject RIV ED - Physiology R&D Projects GA523/08/0111 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000267387800002 Annotation Tumor necrosis factor alpha -induced protein 6 (TNFAIP6) is essential for formation of the cumulus extracellular matrix and female fertility. We studied its expression in porcine preovulatory follicles. The levels of TNFAIP6 mRNA were low in follicles on day 12 and day 15 of the estrous cycle and at 66 h after eCG stimulation but were significantly increased at 4 h after hCG. The high level of TNFAIP6 expression was maintained until 16 h after hCG stimulation and gradually decreased at 24 and 32 h after hCG. During in vitro culture, FSH/LH-induced TNFAIP6 mRNA was expressed in both OCCs and MGCs in a similar temporal pattern as seen in vivo. We conclude that TNFAIP6 expression in the pig, like other species, increases in preovulatory follicles following the LH(hCG) surge. The OCC and MGC display similar patterns of TNFAIP6 expression under both in vivo and in vitro conditions. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
Number of the records: 1