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The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron
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SYSNO ASEP 0332625 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron Author(s) Pavingerová, Daniela (BC-A) Number of authors 1 Source Title Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134
Roč. 53, č. 4 (2009), s. 797-799Number of pages 3 s. Language eng - English Country NL - Netherlands Keywords plant growth regulators ; tissue culture ; Rhododendron Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000272118600038 DOI 10.1007/s10535-009-0147-3 Annotation The influence of cytokinin thidiazuron (TDZ) and auxin indole-3-acetic acid (IAA) on in vitro shoot organogenesis of fifteen Rhododendron genotypes was investigated and a protocol for high frequency adventitious shoot regeneration from leaf explants was developed. High genotypic variation was observed and regeneration frequencies ranged from 0 to 100 %. Genotype Ovation had the highest number of shoots (26.4 per explant) after 12 weeks on medium with 0.57 uM IAA and 1.20 uM TDZ, but only 65 % of explants regenerated. Catawbiense Grandiflorum had 17.7 shoots per explant and 75 % regeneration on medium with 5.70 uM IAA and 0.45 uM TDZ and Van Werden Poelman had 14.3 shoots per explant and 100 % regeneration on medium with 0 57 uM IAA and 0.45 uM TDZ. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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