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Yeast Vitality Determination Based on Intracellular NAD(P)H Fluorescence Measurement during Aerobic-Anaerobic Transition
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SYSNO ASEP 0332254 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Yeast Vitality Determination Based on Intracellular NAD(P)H Fluorescence Measurement during Aerobic-Anaerobic Transition Title Určení viability kvasinek založené na měření fluorescence intracelulárního NAD(P)H během aerobně anaerobního přechodu Author(s) Kuřec, M. (CZ)
Kuncová, Gabriela (UCHP-M) RID, SAI
Brányik, T. (CZ)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 54, č. 1 (2009), s. 25-29Number of pages 5 s. Language eng - English Country CZ - Czech Republic Keywords continous beer fermentation ; brewing yeast ; spectroscopy Subject RIV CE - Biochemistry R&D Projects GA104/06/1418 GA ČR - Czech Science Foundation (CSF) OC 121 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40720504 - UCHP-M (2005-2011) UT WOS 000265832800004 Annotation In this work a method based on on-line intracellular fluorescence measurement is presented. The intracellular NAD(P)H fluorescence of a yeast suspension is recorded during transition from aerobic to anaerobic conditions and the output signal is evaluated as a measure of yeast vitality (quality). This fluorescence method showed a highly satisfactory correlation with even low dead cell numbers where the acidification power test could not be applied. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2010
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