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Association of ‘Candidatus Phytoplasma asteris’ with yellowing and phyllody of Plantago lanceolata
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SYSNO ASEP 0331800 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Association of ‘Candidatus Phytoplasma asteris’ with yellowing and phyllody of Plantago lanceolata Author(s) Fránová, Jana (BC-A) RID, ORCID
Šimková, Marie (BC-A)Number of authors 2 Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 54, č. 5 (2009), s. 469-472Number of pages 4 s. Language eng - English Country CZ - Czech Republic Keywords symptoms ; electron microscopy ; molecular identification Subject RIV EE - Microbiology, Virology R&D Projects 1QS500510558 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000272119700014 DOI 10.1007/s12223-009-0066-x Annotation Long plantains with symptoms resembling those associated with phytoplasma infection were observed in southern Bohemia. TEM showed phytoplasmas in the sieve cells of affected plants. Association of phytoplasmas with the disease was confirmed by PCR using phytoplasma-specific universal ribosomal primers R16F2n/R16R2. The restriction profiles obtained from digestion of the PCR products with endonucleases AluI, HhaI, and MseI showed that the phytoplasmas infecting long plantains in the Czech Republic were indistinguishable from those belonging to the aster yellows group (subgroup 16SrI-B). Sequence analysis of 1748 bp of the ribosomal operon indicated that the closest related phytoplasma was that associated with ‘Rehmannia glutinosa var. purpurea’, originating also in Bohemia. This is the first report of the natural occurrence of ‘Candidatus Phytoplasma asteris’ in plants of Plantago lanceolata. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
Number of the records: 1