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Improved identification of hordeins by cysteine alkylation with 2-bromoethylamine, SDS-PAGE and subsequent in-gel tryptic digestion
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SYSNO ASEP 0331704 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Improved identification of hordeins by cysteine alkylation with 2-bromoethylamine, SDS-PAGE and subsequent in-gel tryptic digestion Title Vylepšená identifikace hordeinů pomocí alkylace cysteinu 2-bromethylaminu, SDS-PAGE a následnému in-gel štěpení Author(s) Řehulková, Helena (UIACH-O)
Marchetti-Deschmann, M. (AT)
Pittenauer, E. (AT)
Allmaier, G. (AT)
Řehulka, Pavel (UIACH-O)Number of authors 5 Source Title Journal of Mass Spectrometry. - : Wiley - ISSN 1076-5174
Roč. 44, č. 11 (2009), s. 1613-1621Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords cysteine alkylation ; 2-bromoethylamine (BEA) ; hordeins ; protein identification Subject RIV CB - Analytical Chemistry, Separation R&D Projects 1M0570 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 1M06030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40310501 - UIACH-O (2005-2011) UT WOS 000272150300009 DOI 10.1002/jms.1675 Annotation The improvement of proteomic identification of hordeins (major storage protein of barley) is described. The approach is based on the increase of number of cleavage sites by chemical modification of cysteines with 2-bromoethylamine. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2010
Number of the records: 1