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Complementation analysis of triple gene block of Potato virus S (PVS) revealed its capability to support systemic infection and aphid transmissibility of recombinant Potato virus X
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SYSNO ASEP 0330919 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Complementation analysis of triple gene block of Potato virus S (PVS) revealed its capability to support systemic infection and aphid transmissibility of recombinant Potato virus X Author(s) Matoušek, Jaroslav (BC-A) RID, ORCID
Schubert, J. (DE)
Dědič, P. (CZ)Number of authors 3 Source Title Virus Research. - : Elsevier - ISSN 0168-1702
Roč. 146, č. 1 (2009), s. 81-88Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords Recombinant virus ; Virus host range ; Potato virus X ; Potato virus S Subject RIV EE - Microbiology, Virology R&D Projects QF3109 GA MZe - Ministry of Agriculture (MZe) 1QS500510558 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000272098700011 DOI 10.1016/j.virusres.2009.09.003 Annotation Specific differences were found in triple gene block (TGB) sequences derived from isolates of ordinary Potato virus S (PVS-O) and Chenopodium-systemic (PVS-CS). In order to investigate functional divergence of PVS TGB variants, they were propagated in chimeric Potato virus X (PVX). Both PVS TGB variants partly complemented PVX TGB, but multiplied to lower titer than the wild-type PVX in N. benthamiana showing attenuated symptoms. From the propagation of recombinant PVX variants in different host and non-host plant species we assume there are additional determinants outside the TGB. Transmission by the aphid Myzus persicae in the recombinant virus was obtained by replacing PVX TGB by TGB from the PVS-CS isolate. These results show the potential function of Carlavirus TGB in aphid transmissibility and underlines the possible biological risks from certain recombinant virus variants. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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