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Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering
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SYSNO ASEP 0330578 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering Title Cholesterolem modifikovaný superporezní poly(2-hydroxyethyl methacrylátový konstrukt pro tkáňové inženýrství Author(s) Kubinová, Šárka (UEM-P) RID, ORCID
Horák, Daniel (UMCH-V) RID, ORCID
Syková, Eva (UEM-P) RIDSource Title Biomaterials. - : Elsevier - ISSN 0142-9612
Roč. 30, č. 27 (2009), s. 4601-4609Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords cell adhesion ; cell viability ; hydrogel Subject RIV FH - Neurology R&D Projects KAN200520804 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50390512 - UEM-P (2005-2011) AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000269330400019 DOI 10.1016/j.biomaterials.2009.05.007 Annotation Modifications of poly(2-hydroxyethyl methacrylate) (PHEMA) with cholesterol and laminin have been developed to design scaffolds that promote cell–surface interaction. Cholesterol- (P(HEMA–CHLMA)) and laminin-modified (LN–PHEMA) superporous PHEMA scaffolds have been prepared by the bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA), cholesterol methacrylate (CHLMA) and the cross-linking agent ethylene imethacrylate in the presence of ammonium oxalate crystals to introduce interconnected superpores in the matrix. Neat PHEMA and laminin-modified PHEMA scaffolds facilitated MSC attachment, but did not support cell spreading and proliferation; the viability of the attached cells decreased with time of cultivation. In contrast, MSCs spread and proliferated on P(HEMA–CHLMA) and LN-P(HEMA–CHLMA) hydrogels. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2010
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