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Gene Content and Virtual Gene Order of Barley Chromosome 1H1,[C],[W],[OA]
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SYSNO ASEP 0330512 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Gene Content and Virtual Gene Order of Barley Chromosome 1H1,[C],[W],[OA] Title Obsah genů a virtuální pořadí genů na chromozómu 1H ječmene Author(s) Mayer, K. F. X. (DE)
Taudien, S. (DE)
Martis, M. (DE)
Šimková, Hana (UEB-Q) RID, ORCID
Suchánková, Pavla (UEB-Q) RID
Gundlach, H. (DE)
Wicker, T. (CH)
Petzold, A. (DE)
Felder, M. (DE)
Steuernagel, B. (DE)
Scholz, U. (DE)
Graner, A. (DE)
Platzer, M. (DE)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Stein, N. (DE)Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 151, č. 2 (2009), s. 496-505Number of pages 10 s. Language eng - English Country US - United States Keywords WHOLE-GENOME AMPLIFICATION ; RICE GENOME ; DNA Subject RIV EB - Genetics ; Molecular Biology R&D Projects LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000270389500002 DOI 10.1104/pp.109.142612 Annotation Chromosome 1H (approximately 622 Mb) of barley (Hordeum vulgare) was isolated by flow sorting and shotgun sequenced by GSFLX pyrosequencing to 1.3-fold coverage. Fluorescence in situ hybridization and stringent sequence comparison against genetically mapped barley genes revealed 95% purity of the sorted chromosome 1H fraction. Sequence comparison against the reference genomes of rice (Oryza sativa) and sorghum (Sorghum bicolor) and against wheat (Triticum aestivum) and barley expressed sequence tag datasets led to the estimation of 4,600 to 5,800 genes on chromosome 1H, and 38,000 to 48,000 genes in the whole barley genome. Conserved gene content between chromosome 1H and known syntenic regions of rice chromosomes 5 and 10, and of sorghum chromosomes 1 and 9 was detected on a per gene resolution. Informed by the syntenic relationships between the two reference genomes, genic barley sequence reads were integrated and ordered to deduce a virtual gene map of barley chromosome 1H. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
Number of the records: 1