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Detection of single nucleotide polymorphisms in p53 mutation hotspots and expression of mutant p53 in human cell lines using an enzyme-linked electrochemical assay
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SYSNO ASEP 0330292 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Detection of single nucleotide polymorphisms in p53 mutation hotspots and expression of mutant p53 in human cell lines using an enzyme-linked electrochemical assay Title Detekce jednonukleotidových polymorfismů v p53 hotspot mutacích a exprese mutantní p53 v lidských buněčných liniích použitím elektrochemické assay spojené s enzymem Author(s) Horáková Brázdilová, Petra (BFU-R)
Šimková, Eva (BFU-R)
Vychodilová, Zdenka (BFU-R)
Brázdová, Marie (BFU-R) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDSource Title Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 21, č. 15 (2009), s. 1723-1729Number of pages 7 s. Language eng - English Country DE - Germany Keywords enzyme-linked electrochemical assay ; SNP typing ; p53 mutation Subject RIV AQ - Safety, Health Protection, Human - Machine R&D Projects GA203/07/1195 GA ČR - Czech Science Foundation (CSF) IAA400040901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC06035 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50040507 - BFU-R (2005-2011) AV0Z50040702 - BFU-R (2007-2013) UT WOS 000268790300010 DOI 10.1002/elan.200904656 Annotation The enzyme-linked electrochemical methode for single nucleotide polymorphism (SNP) typing in the p53 tumor suppressor gene is based on a DNA polymerase-catalyzed extension of a primer hybridized to a target DNA strand by one nucleotide bearing a biotin tag, binding of the streptavidin-alkaline phosphatase conjugate and electrochemical detection of product of enzymatic reaction. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2010
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