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Housekeeping gene selection for Real Time-PCR normalization in female hop (Humulus lupulus L) tissues
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SYSNO ASEP 0330271 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Housekeeping gene selection for Real Time-PCR normalization in female hop (Humulus lupulus L) tissues Author(s) Maloukh, L. (BE)
Matoušek, Jaroslav (BC-A) RID, ORCID
Van Bockstaele, E. (BE)
Roldán-Ruiz, I. (BE)Number of authors 4 Source Title Journal of Plant Biochemistry and Biotechnology. - : Springer - ISSN 0971-7811
Roč. 18, č. 1 (2009), s. 53-58Number of pages 6 s. Language eng - English Country IN - India Keywords gene expression normalization ; hop Humulus lupulus ; Real Time PCR Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000263489400007 Annotation The variability of expression six genes was estimated in leaves and female inflorescences of two hop cultivars (White Golding and Admiral) at different developmental stages by RT-PCR. The combination of the three housekeeping genes DRH1, GAPDH and 7SL-RNA as internal standards for hop, provided reliable results in the quantitative analysis of the transcript accumulation of Hua Enhancer 1 transcription factor (HEN1) homologue in hop tissues and is recommended for future studies of gene expression in female hop tissues. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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