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AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes

SYSNO ASEP	0329884
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes
Author(s)	Kalous, Jaroslav (UZFG-Y)_{RID, ORCID} Kubelka, Michal (UZFG-Y)_{RID, ORCID} Šolc, Petr (UZFG-Y)_{RID, ORCID} Šušor, Andrej (UZFG-Y)_{RID, ORCID} Motlík, Jan (UZFG-Y)_{RID, ORCID}
Source Title	Reproduction. - : BioScientifica - ISSN 1470-1626 Roč. 138, - (2009), s. 645-654
Number of pages	10 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	protein kinase ; porcine oocyte ; oocyte maturation
Subject RIV	CE - Biochemistry
R&D Projects	GA204/06/1297 GA ČR - Czech Science Foundation (CSF)
	GA523/03/0857 GA ČR - Czech Science Foundation (CSF)
	GA524/07/1087 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000270806100004
DOI	10.1530/REP-08-0461
Annotation	The aim of this study was to investigate the involvement of the serine/threonine protein kinase AKT (also called protein kinase B) in the control of meiosis of porcine denuded oocytes (DOs) matured in vitro. Western blot analysis revealed that the two principal AKT phosphorylation sites, Ser473 and Thr308, are phosphorylated at different stages of meiosis. In freshly isolated germinal vesicle (GV)-stage DOs, Ser473 was already phosphorylated. After the onset of oocyte maturation, the intensity of the Ser473 phosphorylation increased, however, which declined sharply when DOs underwent GV breakdown (GVBD) and remained at low levels in metaphase I- and II-stage (MI- and MII-stage). In contrast, phosphorylation of Thr308 was increased by the time of GVBD and reached maximum at MI-stage. A peak of AKT activity was noticed around GVBD and activity of AKT declined at MI-stage. To assess the role of AKT during meiosis, porcine DOs were cultured in 50 mikroM SH-6, a specific
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2010

Number of the records: 1