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The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods
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SYSNO ASEP 0329609 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods Title První report o alkaloidním cylindrospermopsinu v ČR, analyza pomocí imunochemických metod a HPLC-MS Author(s) Bláhová, Lucie (BU-J)
Oravec, M. (CZ)
Maršálek, Blahoslav (BU-J) ORCID, SAI
Šejnohová, Lenka (BU-J)
Šimek, Z. (CZ)
Bláha, Luděk (BU-J)Number of authors 6 Source Title Toxicon. - : Elsevier - ISSN 0041-0101
Roč. 53, č. 5 (2009), s. 519-524Number of pages 6 s. Language eng - English Country GB - United Kingdom Keywords cyanotoxin ; cylindrospermopsin ; methods of detection Subject RIV EF - Botanics R&D Projects 1M0571 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA524/08/0496 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z60050516 - BU-J (2005-2011) UT WOS 000264977700007 DOI 10.1016/j.toxicon.2009.01.014 Annotation We report comparison of the immunochemical ELISA determination with the liquid chromatography/ mass spectrometry (LC/MS) method for CYN analyses in the samples from the Czech Republic, Central Europe. Results of the ELISA and LC/MS showed good qualitative agreement. However, concentrations determined by ELISA were systematically higher than concentrations determined by LC/MS. Differences between methods could be attributed to matrix effects (both in ELISA and LC/MS) or ELISA cross-reactivity with other unidentified derivatives of CYN. Our study demonstrates for the first time occurrence of CYN in the Central Europe. Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2010
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