Number of the records: 1

G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex

SYSNO ASEP	0329471
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex
Title	Aktivita G-proteinů v purifikovaných plazmatických membránách, většinové membránové části a nízkodezitních membránách izolovaných z kortexu potkana
Author(s)	Bouřová, Lenka (FGU-C) Stöhr, Jiří (FGU-C) Lisý, Václav (FGU-C) Rudajev, Vladimír (FGU-C)_RID Novotný, Jiří (FGU-C) Svoboda, Petr (FGU-C)_{RID, ORCID}
Source Title	Medical Science Monitor - ISSN 1234-1010 Roč. 15, č. 4 (2009), BR111-BR122
Number of pages	12 s.
Language	eng - English
Country	US - United States
Keywords	rat cerebral cortex ; plasma membrane ; G-protein activity
Subject RIV	CE - Biochemistry
R&D Projects	LC554 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA309/06/0121 GA ČR - Czech Science Foundation (CSF)
	IAA500110606 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
UT WOS	000266193900005
Annotation	This work describe a straightforward method for the isolation of clearly defined plasma membrane preparations from rat brain cortex - Percoll-purified plasma membranes, bulk of plasma membranes and low-density plasma membrane fragments. [35S]GTPγS binding experiments measured in the presence of high concentration of GDP succeeded in the resolution of the agonist-responsive component of total binding. The highest efficacy of baclofen agrees well with the role of GABA and GABA-B receptors in inhibitory signalling of the brain, the order of efficacy of different agonists of opioid receptors was DADLE DAMGO U-69593
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2010

Number of the records: 1