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Structure and function of the PP2A-shugoshin interaction
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SYSNO ASEP 0328951 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Structure and function of the PP2A-shugoshin interaction Author(s) Xu, Z. (US)
Cetin, B. (GB)
Anger, Martin (UZFG-Y) ORCID
Cho, U. S. (US)
Helmhart, W. (AT)
Nasmyth, K. (AT)
Xu, W. (US)Source Title Molecular Cell. - : Cell Press - ISSN 1097-2765
Roč. 35, č. 4 (2009), s. 426-441Number of pages 16 s. Language eng - English Country US - United States Keywords oocytes ; PP2A-Shugoshin ; mitosis Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000269432600007 DOI 10.1016/j.molcel.2009.06.031 Annotation Accurate chromosome segregation during mitosis and meiosis depends od shugoshin proteins that prevent precocious dissociatin of cohesin from centromeres. Shugoshins associate with PP2A, which is trought to dephosphorylate cohesin and thereby prevent cleavage by separase during meiosis I. A crystal structure of a complex between a fragment of human Sgo1 and an AB.C PP1A holoenzyme reveals that Sgo1 forms a homodimerization is a prerequisite for PP2A binding. While hSgo1 interacts only with the AB.C holoenzymes, its relative, Sgo2 interacts with all PP2A forms and may thus lead to dephosphorylation of distinct substrates. Mutant shugoshin proteins defective id the binding of PP2A cannot protect centromeric cohesin from separase during meiosis I or support the spindle assembly checkpoint in yeast. Finally, we provide evidence that PP2A´s recruitment to chromosomes may be sufficied to protect cohesin from separase in mammalian oocytes. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
Number of the records: 1