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Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic
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SYSNO ASEP 0328620 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic Title Molekulární analýza izolátů viru svinutky bramboru z České republiky Author(s) Plchová, Helena (UEB-Q) RID, ORCID
Čeřovská, Noemi (UEB-Q) RID, ORCID
Moravec, Tomáš (UEB-Q) RID, ORCID
Dědič, P. (CZ)Source Title Virus Genes. - : Springer - ISSN 0920-8569
Roč. 39, č. 1 (2009), s. 153-155Number of pages 3 s. Language eng - English Country US - United States Keywords Potato leafroll virus ; Sequencing ; Phylogeny Subject RIV EB - Genetics ; Molecular Biology R&D Projects QH71123 GA MZe - Ministry of Agriculture (MZe) 1M06030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000267299900021 DOI 10.1007/s11262-009-0374-x Annotation The complete genomes of three Czech isolates VIRUBRA 1/045, VIRUBRA 1/046, and VIRUBRA 1/047 of Potato leafroll virus (PLRV) were sequenced and compared with 13 complete sequences of PLRV isolates available in GenBank. Among the Czech isolates, VIRUBRA 1/046 and 1/047 showed the highest nucleotide (nt) identity (98.7%). PLRV was the most conserved virus in both open reading frames (ORFs) 3 and 4. The most variable regions were ORFs 0 and Rap1. Interestingly, isolate VIRUBRA 1/045 significantly differed from the other two Czech isolates in ORFs 0 and 1. Moreover, we identified mutations in the amino acid (aa) sequences, which were specific for the Czech isolates. Phylogenetic analysis based on ORF0 showed that the Czech isolates could be classified in two of the three groupings of the phylogenetic tree obtained. This is the first report on sequence analysis of the genome sequences of PLRV isolates from the Czech Republic. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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