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Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants
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SYSNO ASEP 0328462 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants Title Regulace fosfoenolpyruvátkarboxylasy v rostlinách tabáku infikovaných virem PVYNTN Author(s) Müller, Karel (UEB-Q) RID, ORCID
Doubnerová, V. (CZ)
Synková, Helena (UEB-Q) RID, ORCID
Čeřovská, Noemi (UEB-Q) RID, ORCID
Ryšlavá, H. (CZ)Source Title Biological Chemistry. - : Walter de Gruyter - ISSN 1431-6730
Roč. 390, č. 3 (2009), s. 245-251Number of pages 7 s. Language eng - English Country DE - Germany Keywords biotic stress ; Nicotiana tabacum ; phosphoenolpyruvate carboxylase (PEPC) Subject RIV ED - Physiology R&D Projects GA206/03/0310 GA ČR - Czech Science Foundation (CSF) 1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000264921500005 DOI 10.1515/BC.2009.029 Annotation The effect of viral infection on regulation of phosphoenolpyruvate carboxylase in Nicotiana tabacum L. leaves was studied. The PEPC activity was 3 times higher in infected plant leaves as compared to healthy plants. The reason of an increase of PEPC activity in consequence of PVYNTN infection was studied. The amount of PEPC determined by Western blot analysis or qRT PCR of PEPC mRNA did not differ in the control and PVYNTN infected plants. Changes in post-translational modification of PEPC by phosphorylation were evaluated comparing activity of the native and the dephosphorylated enzyme. The infected plants were characterized by a higher decrease of the enzyme activity after its dephosphorylation. Immunochemical detection of phosphoproteins by Western blot analysis showed more intensive band corresponding to PEPC from the infected material. This strengthens the hypothesis of an infection-related phosphorylation, which could be part of the plants response to pathogen attack. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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