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Response of Ependymal Progenitors to Spinal Cord Injury or Enhanced Physical Activity in Adult Rat
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SYSNO ASEP 0328392 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Response of Ependymal Progenitors to Spinal Cord Injury or Enhanced Physical Activity in Adult Rat Author(s) Čížková, D. (SK)
Nagyová, M. (SK)
Slovinská, L. (SK)
Novotná, I. (SK)
Radoňák, J. (SK)
Čížek, M. (SK)
Mechirová, E. (SK)
Tomori, Z. (SK)
Hlučilová, Jana (UZFG-Y) RID, ORCID
Motlík, Jan (UZFG-Y) RID, ORCID
Sulla, I. (SK)
Vanický, I. (SK)Source Title Cellular and Molecular Neurobiology. - : Springer - ISSN 0272-4340
Roč. 29, 6-7 (2009), s. 999-1013Number of pages 15 s. Language eng - English Country US - United States Keywords Spinal cord injury ; Neural stem cells ; BrdU Subject RIV FH - Neurology R&D Projects MEB0808108 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000268482300027 DOI 10.1007/s10571-009-9387-1 Annotation Ependymal cells (EC) in the spinal cord central canal (CC) are believed to be responsible for the postnatal neurogenesis following pathological or stimulatory conditions. In this study, we have analyzed the proliferation of the CC ependymal progenitors in adult rats processed to compression SCI or enhanced physical activity. To label dividing cells, a single daily injection of Bromo-deoxyuridine (BrdU) was administered over a 14-day-survival period. Systematic quantification of BrdU-positive ependymal progenitors was performed by using stereological principles of systematic, random sampling, and optical Dissector software. The number of proliferating BrdUlabeled EC increased gradually with the time of survival after both paradigms, spinal cord injury, or increased physical activity. In the spinal cord injury group, we have found 4.9-fold (4 days), 7.1-fold (7 days), 4.9-fold (10 days), and 5.6-fold (14 days) increase of proliferating EC in the rostro-caudal regions, 4 mm away from the epicenter. In the second group subjected to enhanced physical activity by running wheel, we have observed 2.1–2.6 fold increase of dividing EC in the thoracic spinal cord segments at 4 and 7 days, but no significant progression at 10–14 days. Nestin was rapidly induced in the ependymal cells of the CC by 2–4 days and expression decreased by 7–14 days post-injury. Double immunohistochemistry showed that dividing cells adjacent to CC expressed astrocytic (GFAP, S100beta) or nestin markers at 14 days. These data demonstrate that SCI or enhanced physical activity in adult rats induces an endogenous ependymal cell response leading to increased proliferation and differentiation primarily into macroglia or cells with nestin phenotype. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
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