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Enrichment of rat oligodendrocyte progenitor cells by magnetic cell sorting
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SYSNO ASEP 0328388 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Enrichment of rat oligodendrocyte progenitor cells by magnetic cell sorting Author(s) Čížková, D. (SK)
Čížek, M. (SK)
Nagyová, M. (SK)
Slovinská, L. (SK)
Novotná, I. (SK)
Jergová, S. (SK)
Radoňák, J. (SK)
Hlučilová, Jana (UZFG-Y) RID, ORCID
Vanický, I. (SK)Source Title Journal of Neuroscience Methods. - : Elsevier - ISSN 0165-0270
Roč. 184, č. 1 (2009), s. 88-94Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords Oligodendrocytes progenitors Lineage ; Magnetic separation Subject RIV FH - Neurology R&D Projects MEB0808108 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000271053400011 DOI 10.1016/j.neumeth.2009.07.030 Annotation In the present study we have used magnetic cell sorting (MACs) technology to generate enriched oligodendroglial cell populations from the embryonic (E16) rat spinal cord. Target cells were separated by positive selection, using specific A2B5 antibody-labeled MicroBeads achieving optimal recovery and high purity of pro-oligodendroglial cells. Based on immunocytochemical analyses for oligodendroglial developmental markers (A2B5, NG2, RIP and MBP) we were able to characterize and quantify oligodendroglial progenitors (OPCs) and mature oligodendroglial cells in: (i) unseparated heterogeneous population of NSCs, or in (ii) antigen–antibody separated NSCs. Our results showed that MACs technology enable us to gain enriched OPCs from heterogeneous population of spinal NSCs, resulting in a 58–61% of mature oligodendrocytes content (MBP+, RIP+) in comparison to 6–12% of oligodendroglial cells acquired from unseparated population. In addition, the enriched OPCs could be cultured in vitro for several >8 passages, giving rise to a high number of newly formed spheres, as well as high expansion potential. These experiments indicate that MACs technology provide a feasible approach for experimental cell enrichment of desired oligodendroglial progeny, which may be used in future trials for cell-based therapies to treat spinal cord injury. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
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