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Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium
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SYSNO ASEP 0328264 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium Title Řešení problému nekrózy vrcholů prýtů u Harpagophytum procumbens změnou typu cytokininu, koncentrace vápníku a bóru v mediu Author(s) Bairu, M. W. (ZA)
Jain, N. (ZA)
Stirk, W. A. (ZA)
Doležal, Karel (UEB-Q) RID, ORCID
van Staden, J. (ZA)Source Title South African Journal of Botany. - : Elsevier - ISSN 0254-6299
Roč. 75, č. 1 (2009), s. 122-127Number of pages 6 s. Language eng - English Country ZA - South Africa Keywords Boron ; Calcium ; Cytokinins Subject RIV EF - Botanics R&D Projects GA522/06/0108 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000263074300016 DOI 10.1016/j.sajb.2008.08.006 Annotation Various experiments were conducted to characterize and control factors affecting shoot-tip necrosis (STN) in Harpagophytum procumbens. Higher cytokinin concentrations increased the incidence of STN and the problem was aggravated by the addition of auxin (IAA) to the multiplication medium. Optimum shoot multiplication was achieved by omitting auxin and using the cytokinin meta-Topolin riboside (mTR). In the presence of auxin, plantlets produced basal callus that interfered with rooting. The quantity of this basal callus was minimal when mTR was used. Increasing the concentration of either calcium or boron prevented the development of necrotic shoots. When the concentration of both elements was increased simultaneously, negative effects on both growth and STN were observed. Using 6 mM Ca in half-strength MS medium was optimum. Rooted plantlets produced, using the optimized protocol, were acclimatized successfully by direct transfer to the greenhouse in a 1: 1 ratio of sand:soil mixture. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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