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Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli
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SYSNO ASEP 0328089 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli Title Produkce cyklodextrin glukanotransferasy buňkami alkalifilních bacilů Author(s) Atanasova, N. (BG)
Kitayska, T. (BG)
Yankov, D. (BG)
Šafaříková, Miroslava (UEK-B) RID, SAI
Tonkova, A. (BG)Source Title Biochemical Engineering Journal. - : Elsevier - ISSN 1369-703X
Roč. 46, - (2009), s. 278-285Number of pages 8 s. Language eng - English Country CH - Switzerland Keywords alkaliphilic bacilli ; immobilization Subject RIV CE - Biochemistry CEZ AV0Z60870520 - UEK-B (2005-2011) UT WOS 000274797800006 Annotation Cells of Bacillus pseudalcaliphilus 20RF and Bacillus pseudalcaliphilus 8SB isolated from Bulgarian habitats, producers of cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19), were immobilized by three different techniques: on two types of polysulphone membranes; entrapped in agar-gel beads containing magnetite and by nano-particles of silanized magnetite covalently bound on the cell surface. The biocatalysts obtained demonstrated the opportunity for a significantly enhanced CGTase production compared to free cells for a long period of time (10 days semicontinuous cultivation) without impact on their mechanical stability. The cell membrane-biocatalysts exhibited the highest enzyme aktivity. Membrane biocatalysts were applied for a direct cyclodextrin production. The results obtained demonstrated the possibility of starch conversion into cyclodextrins by immobilized cells. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2010
Number of the records: 1