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Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation

SYSNO ASEP	0327369
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation
Title	Metabolické inženýrství biosyntetické dráhy L-valinu v Corynebacterium glutamicum, využívající modulaci promotorové aktivity
Author(s)	Holátko, Jiří (MBU-M) Elišáková, Veronika (MBU-M) Prouza, Marek (MBU-M) Sobotka, Miroslav (MBU-M) Nešvera, Jan (MBU-M)_RID Pátek, Miroslav (MBU-M)_{RID, ORCID}
Source Title	Journal of Biotechnology. - : Elsevier - ISSN 0168-1656 Roč. 139, č. 3 (2009), s. 203-210
Number of pages	8 s.
Language	eng - English
Country	NL - Netherlands
Keywords	corynebacterium glutamicum ; valine production ; promoters
Subject RIV	EE - Microbiology, Virology
R&D Projects	GA204/06/0330 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000263742300002
DOI	10.1016/j.jbiotec.2008.12.005
Annotation	The topic of this paper was modulation of expression of the genes involved in the biosynthesis of branched-chain amino acids in Corynebacterium glutamicum. The activity of the promoters P-ilvD (dihydroxyacid dehydratase) and P-ilvE (transaminase B) was up-modulated and the activity of the promoters P-ilvA (threonine deaminase) and P-leuA (isopropylmalate synthase) was down-modulated by site-directed mutagenesis. The previously constructed strain C. glutamicum ilvNM13 with acetohydroxy acid synthase, resistant to inhibition by all three branched-chain amino acids (L-valine, L-isoleucine and L-leucine), was used as a basis to develop a new type of valine producer by genetic engineering. A constructed weak promoter of ilvA (or leuA), which was introduced into the C. glutamicum chromosome via a gene-replacement technique reduced the biosynthetic rate of isoleucine (or leucine), which lowered the mutant growth rate and increased valine production
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2010

Number of the records: 1