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HPLC-fluorescence detection method for determination of key intermediates of the lincomycin biosynthesis in fermentation broth
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SYSNO ASEP 0327357 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title HPLC-fluorescence detection method for determination of key intermediates of the lincomycin biosynthesis in fermentation broth Title Stanovení klíčových prekurzorů linkomycinu v kultivačním médiu pomocí vysokoúčinné kapalinové chromatografie s fluorescenční detekcí Author(s) Kameník, Zdeněk (MBU-M) RID, ORCID
Kopecký, J. (CZ)
Marečková, M. (CZ)
Ulanová, Dana (MBU-M)
Novotná, Jitka (MBU-M)
Pospíšil, Stanislav (MBU-M) RID
Olšovská, Jana (MBU-M)Source Title Analytical and Bioanalytical Chemistry. - : Springer - ISSN 1618-2642
Roč. 393, 6-7 (2009), s. 1779-1787Number of pages 8 s. Language eng - English Country DE - Germany Keywords Lincomycin precursors ; o-Phthaldialdehyde ; Fluorescence detection Subject RIV CB - Analytical Chemistry, Separation R&D Projects GA204/05/0616 GA ČR - Czech Science Foundation (CSF) IAA6020410 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) 2B08064 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000263826100026 DOI 10.1007/s00216-009-2605-3 Annotation The biosynthetic pathway of the clinically important antibiotic lincomycin is not known in details. The precise knowledge of the lincomycin biosynthesis is a prerequisite for generation of improved derivatives by means of combinatorial genetics. Methods allowing determination of the key intermediates are very important tools of the pathway investigation. Two new high-performance liquid chromatography methods with fluorescence detection for determination of lincomycin precursors in fermentation broth of Streptomyces lincolnensis and its lincomycin nonproducing mutants were developed. The first one enables simultaneous analysis of methylthiolincosamide (MTL) and N-demethyllincomycin (NDL), whereas the second one is suitable for 4-propyl-L-proline (PPL) assay. Both methods are based on the pre-column derivatization: MTL and NDL with 4-chloro-7-nitrobenzofurazan; PPL with o-phthaldialdehyde Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2010
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