Number of the records: 1  

Solid-phase/supercritical-fluid extraction for liquid chromatography of phenolic compounds in freshwater microalgae and selected cyanobacterial species

  1. 1.
    SYSNO ASEP0327251
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleSolid-phase/supercritical-fluid extraction for liquid chromatography of phenolic compounds in freshwater microalgae and selected cyanobacterial species
    TitleKombinace extrakce kapalinou v superkritickém stavu s extrakcí na pevné fázi jako vhodná metoda pro kapalinovou chromatografii fenolických látek v sladkovodních řasách a vybraných sinicích
    Author(s) Klejdus, B. (CZ)
    Kopecký, Jiří (MBU-M) ORCID
    Benešová, L. (CZ)
    Vacek, J. (CZ)
    Source TitleJournal of Chromatography A. - : Elsevier - ISSN 0021-9673
    Roč. 1216, č. 5 (2009), s. 763-771
    Number of pages9 s.
    Languageeng - English
    CountryNL - Netherlands
    Keywordsmethanol ; solid-phase extraction ; phenolic compounds
    Subject RIVEE - Microbiology, Virology
    R&D ProjectsGA525/07/0338 GA ČR - Czech Science Foundation (CSF)
    CEZAV0Z50200510 - MBU-M (2005-2011)
    UT WOS000262797100002
    DOI10.1016/j.chroma.2008.11.096
    AnnotationNew method for SPE-SFE extraction of phenolic compounds, using SPE cartridges inserted directly into SFE extraction cell, was developed. Cyclic addition of binary extraction solvent system based on methanol:water (1:1, v/v) was used for extraction at 40MPa and 80 ◦C. The efficiency of the method was tested on major groups of phenolic compounds like benzoic acid derivatives, hydroxybenzaldehydes and cinnamic acid derivatives. The p-hydroxybenzoic, protocatechic, vanillic, syringic, caffeic and chlorogenic acid; 4-hydroxybenzaldehyde and 3,4-dihydroxybenzaldehyde were identified by HPLC-electrospray mass spectrometry in SPE/SFE extracts of acid hydrolyzates of microalga (Spongiochloris spongiosa) and cyanobacterial strains (Spirulina platensis, Anabaena doliolum, Nostoc sp., and Cylindrospermum sp.)
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2010
Number of the records: 1  

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