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Hyphenated ultra high-performance liquid chromatography–Nano Quantity
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SYSNO ASEP 0327080 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Hyphenated ultra high-performance liquid chromatography–Nano Quantity Author(s) Olšovská, Jana (MBU-M)
Kameník, Zdeněk (MBU-M) RID, ORCID
Cajthaml, Tomáš (MBU-M) RID, ORCIDSource Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1216, č. 30 (2009), s. 5774-5778Number of pages 5 s. Language eng - English Country NL - Netherlands Keywords macrolides ; uv detection ; ultra high-perfonmance liquid chromatography Subject RIV EE - Microbiology, Virology R&D Projects 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000268074800022 DOI 10.1016/j.chroma.2009.05.088 Annotation A novel universal aerosol-based detector Nano Quantity Analyte Detector – NQADTM, connected with an ultra-performance liquid chromatography system is described. The detector was employed for detection of selected antibiotic compounds – macrolides (oleandomycin, erythromycin, troleandomycin, clarithromycin and roxithromycin) that are hard to detect using classical UV detectors due to the lack of chromophores. The determined lowest detection limits under isocratic conditions for these compounds ranged from 3.0 to 5.4 μg/mL. The suitability of the detector connected with ultra high-performance liquid chromatography in the gradient mode was tested on a more complex mixture containing 12 antibiotics. The detector exhibited full compatibility under both the elution modes when UHPLC separations were achieved in relatively short run times Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2010
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