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Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods
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SYSNO ASEP 0326498 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods Author(s) Klusák, Vojtěch (UOCHB-X)
Bařinka, C. (US)
Plechanovová, Anna (UOCHB-X)
Mlčochová, Petra (UOCHB-X)
Konvalinka, Jan (UOCHB-X) RID, ORCID
Rulíšek, Lubomír (UOCHB-X) RID, ORCID
Lubkowski, J. (US)Number of authors 7 Source Title Biochemistry. - : American Chemical Society - ISSN 0006-2960
Roč. 48, č. 19 (2009), s. 4126-4138Number of pages 13 s. Language eng - English Country US - United States Keywords reaction mechanism ; QM/MM calculations ; glutamate carboxypeptidase II ; X-ray crystallography ; mutant activity Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects 1M0508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC512 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000266047400012 DOI 10.1021/bi900220s Annotation The peptide hydrolysis by GCPII was investigated in detail by carrying out the kinetic analysis of GCPII(E424A) using N-Ac-Asp-Glu as substrate. The crystal structure of GCPII(E424A) in complex with N-Ac-Asp-Glu was determined at 1.70 A resolution. The experimental data were then complemented by the QM/MM calculations which provided detailed information concerning the GCPII reaction mechanism. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2010
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