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Analysis of insect triacylglycerols using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry

SYSNO ASEP	0326454
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Analysis of insect triacylglycerols using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry
Author(s)	Kofroňová, Edita (UOCHB-X)_RID Cvačka, Josef (UOCHB-X)_{RID, ORCID} Jiroš, Pavel (UOCHB-X) Sýkora, D. (CZ) Valterová, Irena (UOCHB-X)_{RID, ORCID}
Number of authors	5
Source Title	European Journal of Lipid Science and Technology. - : Wiley - ISSN 1438-7697 Roč. 111, č. 5 (2009), s. 519-525
Number of pages	7 s.
Language	eng - English
Country	DE - Germany
Keywords	atmospheric pressure chemical ionization ; bumblebees ; fat body ; NARP-HPLC
Subject RIV	CC - Organic Chemistry
R&D Projects	IAA4055403 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	2B06007 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000266538000014
DOI	10.1002/ejlt.200800228
Annotation	An HPLC non-aqueous reversed-phase separation system was adapted for analyzing insect triacylglycerols (TAG). The method uses two conventional Nova-Pak C18 columns connected in series, for a total length of 45 cm. The mobile phase gradient is mixed from acetonitrile and 2-propanol, and the flow rate is programmed from 1.0 to 0.7 mL/min. TAG are detected by atmospheric pressure chemical ionization mass spectrometry. The method ensures efficient separation of isomers and analysis of high-molecular weight TAG with equivalent chain lengths up to 72. The method performance is demonstrated on analysis of TAG isolated from the fat body of the bumblebee Bombus lucorum.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2010

Number of the records: 1