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Crystallization of carbohydrate oxidase from Microdochium nivale
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SYSNO ASEP 0326272 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Crystallization of carbohydrate oxidase from Microdochium nivale Title Krystalizace karbohydrátové oxidázy z Microdochium nivale Author(s) Dušková, Jarmila (UMCH-V) RID
Dohnálek, Jan (UMCH-V) RID
Skálová, Tereza (UMCH-V) RID
Ostergaard, L. H. (DK)
Fuglsang, C. C. (DK)
Kolenko, Petr (UMCH-V) RID
Štěpánková, Andrea (UMCH-V)
Hašek, Jindřich (UMCH-V) RIDSource Title Acta Crystallographica Section F-Structural Biology and Crystallization Communications. - : Wiley - ISSN 1744-3091
Roč. 65, č. 6 (2009), s. 638-640Number of pages 3 s. Language eng - English Country GB - United Kingdom Keywords carbohydrate oxidase ; crystallization ; data processing Subject RIV CD - Macromolecular Chemistry R&D Projects IAA500500701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA305/07/1073 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000266452100026 DOI 10.1107/S1744309109017643 Annotation Microdochium nivale carbohydrate oxidase was produced by heterologous recombinant expression in Aspergillus oryzae, purified and crystallized. The enzyme crystallizes with varying crystal morphologies depending on the crystallization conditions. Several different crystal forms were obtained using the hanging-drop vapour-diffusion method, two of which were used for diffraction measurements. Diffraction data suitable for structural analysis were collected and the structure was solved by molecular-replacement method. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2010
Number of the records: 1